Caspase-4 Antibody | 3451

Caspase-4 Antibody | 3451 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Caspase-4 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-4.
The immunogen is located within amino acids 20 - 70 of Caspase-4.

Research Area: Apoptosis, Cancer, Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Depending on cell lines or tissues used, other cleavage products may be observed.

Positive Control 1: Cat. No. 1225 - Ramos Cell Lysate

Positive Control 2: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Caspase-4 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Caspase-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-4 Antibody: TX, ICH-2, Mih1/TX, ICEREL-II, ICE (rel) II, ICH2, Caspase-4, Protease ICH-2, CASP-4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.