Caspase-4, human recombinant | 1084

Caspase-4 (also know as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-4 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-4 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. The recombinant active human caspase-4 was expressed in E. coli. The active caspase-4 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).

1084 | Caspase-4 human recombinant DataSheet

Biomolecule/Target: Caspase-4

Synonyms: Caspase-4

Alternates names: Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12--like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 56729

NCBI Gene Symbol: RETN

Gene Source: Human

Accession #: Q9HD89

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): > 5000 units/mg

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: 19.5 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A