26
Caspase-4, human recombinant | 1084
- SKU:
- 26-1084-GEN
- Availability:
- Usually shipped in 5 working days
Description
Caspase-4 (also know as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-4 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-4 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. The recombinant active human caspase-4 was expressed in E. coli. The active caspase-4 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).
1084 | Caspase-4 human recombinant DataSheet
Biomolecule/Target: Caspase-4
Synonyms: Caspase-4
Alternates names: Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12--like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.
Taglines: A cysteine protease involved in apoptosis
NCBI Gene ID #: 56729
NCBI Gene Symbol: RETN
Gene Source: Human
Accession #: Q9HD89
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): > 5000 units/mg
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: 19.5 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A