Caspase-4 Substrate LEVD-AFC | 1109

Ready-to-use fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD. Caspase-4 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

LEVD-AFC is a ready-to-use fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD.

1109 | Caspase-4 Substrate LEVD-AFC DataSheet

Alternate Name/Synonyms: Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic

Appearance: Liquid

Formulation: DMSO ( 1 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Leu-Glu-Val-Asp-AFC

Salt Form: No

Molecular Formula: C₃₂H₄₀F₃N₅O₁₁

Molecular Weight: 727.5

Cell-Permeable?: No

Purity: >99% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A caspase-4 substrate

MDL Number: MFCD01862605

PubChem CID: 44135103

SMILES: CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C

InChi: InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)

InChi Key: FETZKXHXYOHAAN-UHFFFAOYSA-N