Caspase-5 Antibody | 3457

Caspase-5 Antibody | 3457 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (79%)

Immunogen: Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5.
The immunogen is located within amino acids 170 - 220 of Caspase-5.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Depending on cell lines or tissues used, other cleavage products may be observed.

Positive Control 1: Cat. No. 1225 - Ramos Cell Lysate

Positive Control 2: Cat. No. 1286 - P815 Cell Lysate

Positive Control 3: Cat. No. 17-206 - P815 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Caspase-5 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Caspase-5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-5 Antibody: ICH-3, ICEREL-III, ICE (rel) III, ICH3, Caspase-5, Protease ICH-3, CASP-5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.