Caspase-9 Antibody | 6670

Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

6670 | Caspase-9 Antibody DataSheet

Antibody Target: Caspase-9

Target Alternative Name: Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6

Tag Line: Polyclonal Antibody to detect Caspase-9 in human samples

Category: Caspase-9

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: Recombinant protein of human Caspase-9 (Cat # 1089)

Accession #: P55211

Gene ID: 842

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity Purified Antibody

Application: Western blot

Positive Control: Western Blot: Recombinant protein

Application And Usages: Western blot: 1-4 µg/ml.

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified: Not Verified

Flow Cytometry Verified: Not Verified