CASR Antibody | 7785

CASR Antibody | 7785 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR.
The immunogen is located within the first 50 amino acids of CASR.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: CASR antibody is human, mouse and rat reactive. At least two isoforms of CASR are known to exist.

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 101, 120 kDa
Observed: 115, 125 kDa

Validation: N/A

Isoform: N/A

Purification: CASR antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CASR Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CASR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: CASR Antibody: CAR, FHH, FIH, HHC, EIG8, HHC1, NSHPT, PCAR1, GPRC2A, HYPOC1, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, CaSR

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH) -producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1) . It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1) . Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1, 2) . Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3) .