Cattle bHB (Beta-Hydroxybutyric Acid) ELISA Kit | ELK8618

Cattle bHB (Beta-Hydroxybutyric Acid) ELISA Kit | ELK8618 | Gentaur UK, US & Europe Distribution

Alternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 0.48 μg/mL

Standard: 100 μg/mL

Detection Range: 1.57-100 μg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Hydroxybutyric Acid (bHB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Hydroxybutyric Acid (bHB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Hydroxybutyric Acid (bHB) in the samples is then determined by comparing the OD of the samples to the standard curve.