Cattle Bovine ECGFpro1 (complete for blood ECs) | 300-090-B

Cattle Bovine ECGFpro1 (complete for blood ECs) | 300-090-B | Gentaur UK, US & Europe Distribution

Species: Bovine

Host / biotech: Cattle

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Supplements

Category: Specialities

Synonyms: Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS)

Isotype: N/A

Application: Cell Culture

Detection Range: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 µg/ml, optimal concentration with heparin (30 µg/ml) is about 12 µg/ml.

Species Reactivity/Cross reactivity: human cells, mouse cells, rat cells, cattle cells, pig cells

Antigen: N/A

Description: Endothelila cell growth factor (ECGF) or endothelial cell growth supplement (ECGS) is the term used also for a bovine (or porcine) brain extract containing activities promoting growth and maintenance of endothelial cells (T. Maciag, 1972 and 1982). In the early days this extract has employed as a cell culture supplement together with fetal calf serum to promote long-term serial propagation of human and animla endothelial cells. Its use can reduce the requirement for serum, and heparin has been shown to potentiate the mitogenic activity of the extract (PB Gordon, 1983; Glassberg et al., 1982). The preparation can be used also to grow hybridoma cells without feeder cells (Pintus, 1983). The active factors in these brain extracts have been identified as variants of aFGF (Burgesse, 1985). This ECGF product is supplemented with human rec. VEGF-A in physiological concentrations in order to support growth and differentiation of blood endothelial cells in culture.

Purity Confirmation: N/A

Endotoxin: N/A

Formulation: lyophilized (freeze-dried)

Storage Handling Stability: Also stable at 4°C for several weeks it is recommended to store the product below 0°C. After reconstitution the product should be stored in aliquots at -20°C to -70°C.

Reconstituation: Reconstitute the contents of the vial in 2 ml of prewarmed (37°C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.

Molecular Weight: N/A

Lenght (aa): N/A

Protein Sequence: N/A

NCBI Gene ID: N/A