Cattle Hpt (Haptoglobin) ELISA Kit | ELK7354

Cattle Hpt (Haptoglobin) ELISA Kit | ELK7354 | Gentaur UK, US & Europe Distribution

Alternative Names: HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin

Species: Cattle

Assay Type: Sandwich

Sensitivity: 5.9 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Haptoglobin (Hpt) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Haptoglobin (Hpt) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Haptoglobin (Hpt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Haptoglobin (Hpt) in the samples is then determined by comparing the OD of the samples to the standard curve.