Cattle TGFb1 (Transforming Growth Factor Beta 1) ELISA Kit | ELK5467

Cattle TGFb1 (Transforming Growth Factor Beta 1) ELISA Kit | ELK5467 | Gentaur UK, US & Europe Distribution

Alternative Names: TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 4.67 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 1 (TGFb1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the OD of the samples to the standard curve.