Caveolin-1 Antibody | 45-027

Caveolin-1 Antibody | 45-027 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig

Immunogen: The immunogen for this antibody is: C-DELSEKQVYDAH

Research Area: Homeostasis

Tested Application: E, WB, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 22kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5kDa according to NP_001744.2) . Recommended concentration: 0.001-0.01ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Heart and Spleen, and Mouse and Rat Lung for this particular batch.Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_001744.2; NP_001166366.1) . Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: CAV1, caveolin 1, caveolae protein, 22kDa, HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP085

User Note: N/A

BACKGROUND: N/A