CCA1 | Circadian clock associated 1 | AS13 2659

CCA1 | Circadian clock associated 1 | AS13 2659 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Co-immunoprecipitation (Co-IP), Western blot (WB)

Related Products: AS13 2661 | Anti-LHY | Late elongated hypocotyl, rabbit antibodiesAS13 2646 | Anti-TOC1 | TIMING OF CAB EXPRESSION 1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1: 500 (Co-IP), 1 : 500 (WB)

Molecular weight: 67 | 80 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: N/A

Not reactive in: Oryza sativa

Additional Information: Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100°C heating block, not at RT. Centrifugation for 5 minutes will remove cell debris. Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots. Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.

Background: CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G46830

Category: Circadian clock

Research Area: Plant Biology