CCDC99 Antibody | 57-083

CCDC99 Antibody | 57-083 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Monkey

Immunogen: This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human CCDC99.

Research Area: Cell Cycle

Tested Application: WB, IHC-P

Application: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 70 kDa

Validation: N/A

Isoform: N/A

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality: Polyclonal

Clone: N/A

Isotype: Rabbit Ig

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration: batch dependent

Storage Condition: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041}, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99 {ECO:0000255|HAMAP-Rule:MF_03041}, Rhabdomyosarcoma antigen MU-RMS-404A, Spindle apparatus coiled-coil domain-containing protein 1 {ECO:0000255|HAMAP-Rule:MF_03041}, SPDL1 {ECO:0000255|HAMAP-Rule:MF_03041}

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.