CCS | Chloroplastic copper chaperone for SOD | AS07 219

CCS | Chloroplastic copper chaperone for SOD | AS07 219 | Gentaur UK, US & Europe Distribution

Immunogen: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS06 170 | Anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase, rabbit antibodiesAS06 125 | Anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase, rabbit antibodiesAS09 524 | Anti-MnSOD | manganese superoxide dismutase, rabbit antibodiescollection of antibodies to plant stress proteins

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 33.8 | 37 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays

Predicted Reactivity: Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G12520

Category: Heavy metal stress

Research Area: Plant Biology