CD117 Antibody [2B8] (APC-Cy5.5) | 99-021

CD117 Antibody [2B8] (APC-Cy5.5) | 99-021 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: Mouse IL-3-dependent bone marrow mast cells

Research Area: Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

Tested Application: Flow

Application: CD117 Antibody [2B8] for use in flow cytometry assays.

Specificiy: CD117

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [2B8]

Isotype: IgG2b

Conjugate: APC-Cy5.5

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: c-Kit, mast cell growth factor, stem cell factor, steel factor, SCF

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF) . Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.