CD11b Antibody [M1/70] | 98-642

CD11b Antibody [M1/70] | 98-642 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse, Human, Rhesus, Baboon, Rabbit

Homology: N/A

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes

Research Area: Immunology, Neuroscience, Signal Transduction

Tested Application: E, IHC-Fr, ICC, Flow

Application: CD11b Antibody [M1/70] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and blocking assays.

Specificiy: CD11b

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [M1/70]

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: Integrin alphaM, Mac-1alpha, Mac-1, Mo1, CR3, C3biR

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD11b, the integrin alphaM subunit, combines with CD18, the integrin beta2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3) , which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.