CD122 Antibody [5H4] | 99-060

CD122 Antibody [5H4] | 99-060 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-mbetat-28)

Research Area: Other

Tested Application: IHC-Fr, Flow, IP

Application: CD122 Antibody [5H4] for use in flow cytometry and immunoprecipitation assays.

Specificiy: CD122

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [5H4]

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: IL-2Rbeta, IL-15 receptor beta chain

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (iii) the common gamma chain (gammac) that does not bind IL-2. The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding.