CD14 Antibody [5A3H5D5 (FC) / 5A3B11B5] | 32-128

CD14 Antibody [5A3H5D5 (FC) / 5A3B11B5] | 32-128 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3) .

Research Area: Innate Immunity

Tested Application: E, IHC

Application: IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.

Clonality: Monoclonal

Clone: 5A3H5D5 (FC) / 5A3B11B5

Isotype: IgG1

Conjugate: Unconjugated

Physical State: N/A

Buffer: Purified antibody in PBS containing 0.03% sodium azide.

Concentration: N/A

Storage Condition: CD14 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Myeloid cell-specific leucine-rich glycoprotein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP) . LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.