Description
CD28 Antibody [204.12] | 33-494 | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Human, Pig, Bovine, Sheep, Mouse
Homology: N/A
Immunogen: Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
Research Area: Immunology
Tested Application: Flow, IF, Func
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Functional Activity (Order SAF formulation)
Optimal dilution of the CD28 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: 204, 12
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: CD4 Antibody [9H5A8] : CD4mut
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.