CD3e Antibody [C363.29B] | 98-581

CD3e Antibody [C363.29B] | 98-581 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: IL-4 producing Th2 cell lines including D10

Research Area: Immunology

Tested Application: E, Flow

Application: CD3e Antibody [C363.29B] for use in flow cytometry, immunohistochemistry, immunoprecipitation, depletion, CMCD, and activation assays.

Specificiy: CD3epsilon

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [C363.29B]

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in PBS

Concentration: 0.5 mg/mL

Storage Condition: Store vial at -20˚C

Alternate Name: CD3 epsilon

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR) . It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and na��ve T cell in vitro. Immobilized C363.2B9 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining, and a good immunoprecipitating antibody.