Description
CD3e polyclonal Antibody | BS62211 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human,Mouse
Application: WB IHC
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200
Background: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) . Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CD3e polyclonal Antibody detects endogenous levels of CD3e protein.
Molecular Weight: ~ 21 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen=CD3e; CD3E; T3E
Immunogen: Synthetic peptide, corresponding to Human CD3e.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: Tumor Angiogenesis,Contribution of ECM and Cytoskeletal Factors to EMT,