CD4 Antibody [GK1.5] (Biotin) | 98-585

CD4 Antibody [GK1.5] (Biotin) | 98-585 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: Mouse CTL clone V4

Research Area: Immunology

Tested Application: WB, IHC-P, IHC-Fr, ICC, Flow

Application: CD4 Antibody [GK1.5] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and separation assays.

Specificiy: CD4

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [GK1.5]

Isotype: IgG2b

Conjugate: Biotin

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: L3T4, Ly-4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.