CD45.1 Antibody [A20] (APC-Cy7) | 98-923

CD45.1 Antibody [A20] (APC-Cy7) | 98-923 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse

Homology: N/A

Immunogen: SJL mouse thymocytes and splenocytes

Research Area: Immunology

Tested Application: IHC-Fr, ICC, Flow

Application: CD45.1 Antibody [A20] for use in flow cytometry assays.

Specificiy: CD45.1

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [A20]

Isotype: IgG2a

Conjugate: APC-Cy7

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: Ly-5.1, LCA, SJL, T200

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The CD45 antigen is an essential regulator of leukocyte activation and development and is now known to be a protein tyrosine phosphatase, e.g., CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling, and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685, and 1820) and as allelic forms CD45.1 (Ly-5.1) and CD45.2 (Ly-5.2) . The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD45.1 allotype.