CD62L Antibody [MEL-14] (FITC) | 76-202

CD62L Antibody [MEL-14] (FITC) | 76-202 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: N/A

Research Area: Immunology

Tested Application: Flow

Application: N/A

Specificiy: The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L) , a receptor with lectin-like and Epidermal Growth Factor-like domains.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clonality: Monoclonal

Clone: MEL-14

Isotype: Rat IgG2a, kappa

Conjugate: FITC

Physical State: liquid

Buffer: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Concentration: batch dependent

Storage Condition: The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.

Alternate Name: Lnhr, CD62L, Ly-22, Lyam1, Ly-m22, Lyam-1, LECAM-1, AI528707, L-selectin, Sell

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L) , a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin, 74 kDa on lymphocytes or 95 kDa on neutrophils. In the mouse organism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34) . It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules) . In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes. After cell activation, it is quickly shed by neutrophils and lymphocytes.