CD62L Antibody [MEL-14] (PE-Cy5) | 98-823

CD62L Antibody [MEL-14] (PE-Cy5) | 98-823 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: C3H/eb cloned mouse B cell lymphoma 38C-13

Research Area: Immunology

Tested Application: E, IHC-Fr, ICC, Flow, Blocking, IP, Depletion

Application: CD62L Antibody [MEL-14] for use in flow cytometry assays.

Specificiy: CD62L

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [MEL-14]

Isotype: IgG2a

Conjugate: PE-Cy5

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: L-selectin, LECAM-1, LAM-1, Ly-22, Sell, Lnhr, Lyam-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.