CD8a Antibody [3-298] | 99-320

CD8a Antibody [3-298] | 99-320 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken, Turkey

Homology: N/A

Immunogen: Chicken thymocytes

Research Area: Cancer, Immunology, Signal Transduction

Tested Application: IHC, ICC, Flow

Application: CD8a Antibody [3-298] for use in flow cytometry, immunohistochemistry, western blotting, immunoprecipitation, and separation assays.

Specificiy: CD8alpha

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [3-298]

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: In the chicken the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. The monoclonal antibody 3-298 recognizes the CD8alpha chain of both the chicken and turkey.