CDNF, Human Recombinant | 7127

CDNF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. CDNF and its structural homolog MANF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. CDNF and MANF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human CDNF is an 18.5 kDa protein consisting of 162 amino acids including 8 cysteine residues.

7127 | CDNF Human Recombinant DataSheet

Biomolecule/Target: CDNF

Synonyms: Cerebral dopamine neurotrophic factor, ARMETL1

Alternates names: BMP-12/GDF-7, BMP12/GDF7, BMP 12/GDF 7,

Taglines: Promotes survival, growth and function of dopamine specific neurons

NCBI Gene ID #: 151449

NCBI Gene Symbol: BMP12/GDF7

Gene Source: Human

Accession #: BAD07014

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 µg/ml.

Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by ADTC5 mouse chondrogenic cells. The ED for this effect is typically 0.2 – 2.5 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 27 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A