Description
CDNF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. CDNF and its structural homolog MANF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. CDNF and MANF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human CDNF is an 18.5 kDa protein consisting of 162 amino acids including 8 cysteine residues.
7127 | CDNF Human Recombinant DataSheet
Biomolecule/Target: CDNF
Synonyms: Cerebral dopamine neurotrophic factor, ARMETL1
Alternates names: BMP-12/GDF-7, BMP12/GDF7, BMP 12/GDF 7,
Taglines: Promotes survival, growth and function of dopamine specific neurons
NCBI Gene ID #: 151449
NCBI Gene Symbol: BMP12/GDF7
Gene Source: Human
Accession #: BAD07014
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 µg/ml.
Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by ADTC5 mouse chondrogenic cells. The ED for this effect is typically 0.2 2.5 µg/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 27 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.
Amino acid sequence: N/A