Description
CELA3B Antibody [CELA3B/1257] | 33-782 | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
Research Area: Cell Cycle, Signal Transduction
Tested Application: WB, IHC-P, IF, E, Flow
Application: ELISA: 2-4 ug/ml; order BSA free format for coating
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC-P: 1-2 ug/ml for 30 min at RT
Optimal dilution of the Elastase 3B antibody should be determined by the researcher.
1. FFPE staining requires
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G
Clonality: Monoclonal
Clone: CELA3B/1257
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.