CELA3B Antibody [CELA3B/1757] | 33-794

CELA3B Antibody [CELA3B/1757] | 33-794 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody.

Research Area: Cell Cycle, Signal Transduction

Tested Application: WB, E, IHC-P, IF, Flow

Application: Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
ELISA: 2-4 ug/ml; order BSA free formulation for coating
IHC (FFPE) : 1-2 ug/ml for 30 min at RT
Optimal dilution of the CELA3B antibody should be determined by the researcher.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: CELA3B/1757

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B) , or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.