Description
Centromere Protein F Antibody | 45-393 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Cow
Immunogen: The immunogen for this antibody is: GLESKGSENCKVQ
Research Area: Cancer
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Specificiy: The immunizing peptide shows one mismatch with the corresponding sequence of the NCBI-curated isoform NP_057427.3.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: CENPF, centromere protein F (350/400kD, mitosin) , CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD) , cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400ka (mitosin) , RP11-262H5.1, hcp-1, centromere protein F, mitosin
User Note: N/A
BACKGROUND: N/A