cFBPase | Cytosolic fructose-1, 6-bisphosphatase (cytoplasm marker in photosynthetic tissues) | AS04 043

cFBPase | Cytosolic fructose-1, 6-bisphosphatase (cytoplasm marker in photosynthetic tissues) | AS04 043 | Gentaur UK, US & Europe Distribution

Immunogen: Overexpressed cytosolic fructose 1, 6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase UniProt: Q9MA79, TAIR: AT1G43670

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: Collection of other antibodies which can be used as markers of cytoplasmPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 5 000 (WB)

Molecular weight: 45 | 37 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Brassica napus, Macroptilium atropurpureum, Nicotiana benthamiana, Pinus silvestris, Oryza sativa, Petunia hybrida cv. Mitchell, Solanum tuberosum, Zea mays cellular compartment marker of cytoplasm in photosynthetic tissues

Predicted Reactivity: Capsella rubella, Pisum sativum, Ricinus communis, Glycine max, Phaseolus vulgaris, Sesamum indicum, Spinacia oleracea, Populus trichocarpa, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii

Additional Information: This antibody does not react with chloroplastic form of FBPase.Will this antibody be good as a cytosolic (non-microsomal control) in Arabidopsis thaliana roots? Although it has never been tested there is every likelihood that cFBPase will be expressed at reasonable levels even in roots. Even though the biosynthetic flux through to Sucrose may not be high as in mesophyll cells, central metabolism will still be active in young roots and the Sucrose etc being supplied externally still needs to be utilised.

Background: Fructose-1, 6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1, 6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1, 6-bisphosphate 1-phosphohydrolase

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G43670

Category: Carbohydrates

Research Area: Plant Biology, Photosynthesis