CFTR Antibody [CFTR1-1] | 34-037

CFTR Antibody [CFTR1-1] | 34-037 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Human recombinant protein was used as the immunogen for this CFTR antibody.

Research Area: Signal Transduction

Tested Application: IF, WB, IHC-P

Application: Immunofluorescence: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE) : 0.5-1 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: CFTR1-1

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Cystic fibrosis transmembrane conductance regulator (CFTR) is composed of two membrane-spanning domains (MSD) , two nucleotide-binding domains (NBD) , and an R domain. It is structurally similar to multidrug resistance (MDR1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The protein is a small conductance adenosine 3', 5'-cyclic monophosphate (cAMP) -activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.