CheKine™ Coenzyme Ⅰ NAD (H) Colorimetric Assay Kit | KTB1020

CheKine™ Coenzyme Ⅰ NAD (H) Colorimetric Assay Kit | KTB1020 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

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Kit Component: • Extraction Buffer
• Assay Buffer I
• Assay Buffer II
• Substrate Mix
• Enzyme Mix

Features & Benefits : • Determination of NADK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.

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Usage Note: • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Substrate Mix and Enzyme Mix must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25°C (general species) or 37°C (mammal) .

Storage Conditions: Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

Shipping: Gel pack with blue ice.

Background: NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the phosphorylation reaction of NAD (H) with ATP or inorganic polyphosphate [poly (P) ] as a phosphoryl donor to generate NADP (H) . Therefore, NAD kinase plays an important role in the synthesis of NADP (H) and the regulation of the balance between NAD (H) and NADP (H) .

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Tag: NADK