CheKine™ Glutathione Peroxidase (GSH-Px) Activity Colorimetric Assay Kit | KTB1640

CheKine™ Glutathione Peroxidase (GSH-Px) Activity Colorimetric Assay Kit | KTB1640 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

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protein Lenght: N/A

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Kit Component: • Assay Buffer
• Chromogen
• TrxR Cofactor

Features & Benefits : • Determination of TrxR activity in serum, plasma, tissue/cell lysates and other biological fluids.

Molecular Weight: N/A

Usage Note: • The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
• In the detection of TrxR activity in cells, the cell number must be between 3-5 x 106, and the extraction of TrxR in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: TrxR is a NADPH-dependent dimer selenase that includes the FAD domain. It belongs to the pyridine nucleotide-disulfide redoxase family and forms a thioredoxin system with thioredoxin and NADPH. TrxR has similar activity to GR and catalyzes the reduction of GSSG to GSH, which is one of the key enzymes in the glutathione redox cycle.

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Tag: TrxR