CheKine™ Glutathione Reductases (GR) Activity Colorimetric Assay Kit | KTB1620

CheKine™ Glutathione Reductases (GR) Activity Colorimetric Assay Kit | KTB1620 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

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protein Lenght: N/A

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Kit Component: • Assay Buffer
• Chromogen
• Substrate

Features & Benefits : • Determination of GST activity in serum, plasma, tissue/cell lysates and other biological fluids.
• A broad range linearity: 2-76 U/L.
• Sensitivity down to 2 U/L.

Molecular Weight: N/A

Usage Note: • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The sample preparation should be carried out on ice.
• If the experiment is not carried out immediately, the samples can be kept at -80oC for 1 month.
• In the detection of GST activity in cells, the cell number must be between 3-5 x 106, and the extraction of GST in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
• Pre-test is needed before experiment, two or three samples with large difference should be diluted into different concentrations to test.
• If the results collected within 5 minutes are not linear, or the sample absorbance value is greater than 1, it is suggested that the sample should be diluted with distilled water and the result should be multiplied by the dilution factor.
• The reaction temperature has influence on the result. Keep the temperature at 25°C (for general species) or 37°C (for mammals) .

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble. The glutathione conjugatesare metabolized further to mercapturic acid and then excreted. GST has the activity of GSH-Px, also called non-Se GSH-Px, which has the function of repairing oxidative damage of macromolecule such as DNA, protein and so on. GST catalyzed reaction decreased GSH content, but did not increase GSSG content. Based on their sequence homology, substrate specificity and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes. These classes are comprised of both cytosolic and microsomal enzymes.

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Tag: GST