CheKine™ NADH Oxidase (NOX) Colorimetric Assay Kit | KTB1021

CheKine™ NADH Oxidase (NOX) Colorimetric Assay Kit | KTB1021 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

Activity: N/A

protein Lenght: N/A

Preparation: N/A

Purity: N/A

Formulation: N/A

Kit Component: • Extraction Buffer
• Assay Buffer
• Substrate Mix
• LDH

Features & Benefits : • Determination of Pyruvate kinase (PK) activity in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.
• Kit components and procedures are simple.
• Sample preparation, experimental steps and result calculation are more detailed and accurate.
• Long shelf life.

Molecular Weight: N/A

Usage Note: • Assay kit is intended for research use only. Not for use in diagnostic procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
• Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before assay.
• 96-well UV microplate is needed
• Sample preparation and other processes need to be performed on ice, and the enzyme activity must be measured on the same day and avoid repeated freezing and thawing to prevent enzyme inactivation.
• LDH must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25℃ (general species) or 37℃ (mammal) .

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: PK (EC 2.7.1.40) is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the last step reaction in the glycolysis process. It is one of the main rate-limiting enzymes in the glycolysis process and the key to ATP production. It is important to measure PK activity.

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Tag: PK