CheKine™ Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit | KTB1122

CheKine™ Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit | KTB1122 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

Activity: N/A

protein Lenght: N/A

Preparation: N/A

Purity: N/A

Formulation: N/A

Kit Component: • Extraction Buffer
• Assay Buffer
• GR
• GR Cofactor
• Chromogen
• Standard

Features & Benefits : • Determination of the content of total glutathione in a variety of biological samples such as tissues, bacteria, fungus, cells, serums, ect.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.

Molecular Weight: N/A

Usage Note: • Do not mix or substitute reagents or materials from other kit lots or different vendors, or it may cause abnormal result.
•Avoid foaming or bubbles when mixing or reconstituting components.
• To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
•Ensure all reagents and solutions are at the appropriate temperature before starting the assay.
•Pls dilute the sample furtherly when the concentration of sample is higher than the maximal concentration of standard.

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: Glutathione is a tripeptide consisting of glutamate, cysteine and glycine. Glutathione contains sulfhydryl groups and has antioxidant and integrated detoxification effects. Glutathione has reduced form (GSH) or oxidized form (GSSG), reduced glutathione predominates in physiological conditions. Glutathione reductase catalyzes the alternations between the two forms. GSH/GSSG is one of the most important redox pairs in cells. Therefore, the determination of GSH and GSSG content and GSH/GSSG ratio in cells can well reflect the redox state of cells and is also one of the main indicators of the glutathione redox cycle.

Alternative Names: Toal Glutathione; T-GSH; t-GSH

Search Names: Toal Glutathione; T-GSH; t-GSH

Tag: T-GSH