CheKine™ Superoxide Dismutases (SOD) Activity Colorimetric Assay Kit | KTB1030

CheKine™ Superoxide Dismutases (SOD) Activity Colorimetric Assay Kit | KTB1030 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

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protein Lenght: N/A

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Kit Component: • Assay Buffer
• Sample Diluent
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate

Features & Benefits : • Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining catalase activity directly by utilizing the peroxidatic function of catalase, which can not be interfered by other peroxidases.
• A broad range linearity: 2-75 µM.
• Measure catalase activity down to 2 U/ml

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Usage Note: • If not assayed immediately, samples can be stored at -80°C.
• Overheating can inactivate catalse. The enzyme should be kept cold during sample preparation and assaying.
• In general, catalase is very unstable at high dilution. It is recommned to store samples concentrated and assay within 30 minutes afrer dilution.

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: Catalase (EC 1.11.1.6), is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen, ubiquitously present in aerobic cells containing a cytochrome system. Hydrogen peroxide is highly deleterious to the cell and its accumulation will cause oxidation of cellular targets such as DNA, proteins, and lipids, thus leading to mutagenesis and cell death. Removal of the hydrogen peroxide (H2O2) from cells by using catalase provides protection against oxidative damage to the cell. The role of catalase in oxidative stress related diseases has been widely studied. Catalase also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. Aliphatic alcohols are specific substrates for catalase, however, other enzymes with peroxidatic activity do not utilize these substrates.

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Tag: CAT