CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit | KTB1650

CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit | KTB1650 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

Activity: N/A

protein Lenght: N/A

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Kit Component: • Assay Buffer
• Substrate
• H2O2

Features & Benefits : • Determination of TPX activity in serum, plasma, tissue/cell lysates and other biological fluids.

Molecular Weight: N/A

Usage Note: • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
• In the detection of TPX activity in cells, the cell number must be between 3-5 x 106, and the extraction of TPX in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.

Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: TPX belongs to the family of peroxidases. It mainly achieves anti-oxidation by reducing hydrogen peroxide and some hydroperoxides in the body. It functions similarly to GPX and is one of the key enzymes in the glutathione redox cycle. TPX is ubiquitous in various organisms, such as yeast, plants, animals, protozoa, parasites, bacteria, and archaea, and is highly conservative in evolution. TPX is closely related to the regulation of cell proliferation, differentiation, apoptosis and tumorigenesis. The main functions of TPX include cell detoxification, anti-oxidation, and regulation of signal transduction and immune response mediated by hydrogen peroxide.

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Tag: TPX