Chicken SEPP1 (Selenoprotein P1, Plasma) ELISA Kit | ELK9490

Chicken SEPP1 (Selenoprotein P1, Plasma) ELISA Kit | ELK9490 | Gentaur UK, US & Europe Distribution

Alternative Names: SEPP; SeP; SEP-P1; SELP

Species: Chicken

Assay Type: Sandwich

Sensitivity: 29.6 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenoprotein P1, Plasma (SEPP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Selenoprotein P1, Plasma (SEPP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenoprotein P1, Plasma (SEPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenoprotein P1, Plasma (SEPP1) in the samples is then determined by comparing the OD of the samples to the standard curve.