Chlorpyrifos (polyclonal antibody) | AS13 2736

Chlorpyrifos (polyclonal antibody) | AS13 2736 | Gentaur UK, US & Europe Distribution

Immunogen: BSA-conjugated Chlorpyrifos, Target: Chlorpyrifos, CAS no.: 2921-88-2 purchased from SIGMA

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG, purified by caprylic acid extraction.

Format: Liquid

Tested Application: ELISA (ELISA), Immuno-affinity-chromatography (IAC)

Related Products: AS19 4363 | Chlorpyrifos (antigen), trial sampleSecondary antibodies

Recommended Dilutions: 1 : 500 (ELISA)

Molecular weight: N/A

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: ELISA titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested in this technique. Plates are coated with 400 ng/ml ovalbumin conjugated-chlorpyrifos. Detection antibody is: anti-rabbit IgG, HRP-conjugated antibody in dilution 1: 8000. The titer in the indirect ELISA using OVA-conjugate was 500, in the indirect ELISA it was 100 000. The antibody is highly specific for chlorpyriphos showing no cross-reactivity with related and unrelated compounds. In the direct ELISA the detection limit was 1-4 nM (0.3-1.3 µg/L) . Affinity and avidity were not determined. Specific antibody concentration is not determined as it is provided as a total immunoglobulin pool (purified by caprilic acid) . Caprilic acid purified immunoglobulins can be used for most procedures like: coating plates for antigen capture assays and preparation of immunoaffinity columns. However, such preparations are not suitable for: conjugation with radioisotopes, enzymes, and biotin where contaminating proteins will reduce the specific activity..

Background: Chlorpyrifos is an insecticide belonging to the group of organophosphothioates. It is used in the culture of vegetables and in citrus orchards. Chlorpyrifos is an acetylcholinesterase inhibitor and as such having a highly toxic effect on the nervous system. There is evidence that chlorpyrifos has an effect on the foetus, which may lead to behavioural disorders. Chlorpyrifos, CAS no.: 2921-88-2, Solubility: Methanol, Iso-octanol, Benzene.

Reconstitution: N/A

Storage: Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.

TAIR Nnumbre: N/A

Category: Insecticides

Research Area: Toxins