Description
Chymase 2 Antibody | 46-865 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Mouse
Immunogen: The immunogen for this antibody is: CKIFKHYKDSLQ
Research Area: Homeostasis
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 26.9kDa according to NP_001019885.1 and NP_034912.3. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: Mcp 10 and Mcp 9 (GeneID 545055) are almost identical in their protein sequence. No cross-reactivity expected with the similar Mcp4.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: Cma2, Chymase 2, mast cell, Mast cell protease 10, Mcp10,
User Note: N/A
BACKGROUND: N/A