CLU Antibody (N-term) [APG02671G]

CLU Antibody (N-term) [APG02671G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This CLU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CLU.

Cellular Localisation: [Isoform 1]: Secreted. Note=Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. [Isoform 6]: Cytoplasm. Note=Keeps cytoplasmic localization in stressed and unstressed cell.

Molecular Weight: 52495

Clone: Polyclonal

Gene Name: CLU (HGNC:2095)

Gene ID: 1191

Function: [Isoform 1]: Functions as extracellular chaperone that prevents aggregation of non native proteins (PubMed:11123922, PubMed:19535339) . Prevents stress-induced aggregation of blood plasma proteins (PubMed:11123922, PubMed:12176985, PubMed:17260971, PubMed:19996109) . Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed:12047389, PubMed:17412999, PubMed:17407782) . Does not require ATP (PubMed:11123922) . Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70 (PubMed:11123922) . Does not refold proteins by itself (PubMed:11123922) . Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed:21505792) . Protects cells against apoptosis and against cytolysis by complement (PubMed:2780565) . Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:20068069) . Promotes proteasomal degradation of COMMD1 and IKBKB (PubMed:20068069) . Modulates NF-kappa-B transcriptional activity (PubMed:12882985) . A mitochondrial form suppresses BAX- dependent release of cytochrome c into the cytoplasm and inhibit apoptosis (PubMed:16113678, PubMed:17689225) . Plays a role in the regulation of cell proliferation (PubMed:19137541) . An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5 (PubMed:22689054) . Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed:24073260) . Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity) . Plays a role in the clearance of immune complexes that arise during cell injury (By similarity) .

Summary: Tissue Location: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level) . Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC, IHC-P-Leica

Dilution: WB--1:1000

Synonyms: Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, TRPM-2, Clusterin beta chain, ApoJalpha, Complement cytolysis inhibitor a chain, Clusterin alpha chain, ApoJbeta, Complement cytolysis inhibitor b chain, CLU, APOJ, CLI, KUB1