Control Normal llama IgG1 | XR-8002

Control Normal llama IgG1 | XR-8002 | Gentaur UK, US & Europe Distribution

Host : Llama

Reactivity : N/A

Tested Application : E, WB, Flow

Application: Llama IgG1 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Isotype: IgG1

Physical State: Liquid

Buffer: 0.075 M Tris, 0.375 M NaCl, 25% glycerol, 0.01% (w/v) Sodium Azide

Concentration: 1 mg/mL

Storage Condition: Store vial at -20˚C prior to opening. Aliquot contents and freeze at -20˚C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use.

Background: Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35% - 70% of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses. All gamma chain camelid antibodies exhibiting the more conventional assembly of two light and two heavy chains with molecular weight ~150 kDa, belong to the IgG1 subclass.