Control Normal llama IgG2 (biotin) | XR-8016

Control Normal llama IgG2 (biotin) | XR-8016 | Gentaur UK, US & Europe Distribution

Host : Llama

Reactivity : N/A

Tested Application : E, WB, Flow

Application: Llama IgG2 Biotin conjugated isotype control can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

Isotype: IgG2

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Concentration: 1 mg/mL

Storage Condition: Store vial at 4˚C prior to restoration. For extended storage aliquot contents and freeze at -20˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Restore with 100 uL deionized water (or equivalent)

Background: Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.