Control Normal llama IgG2 (FITC) | XR-8008

Control Normal llama IgG2 (FITC) | XR-8008 | Gentaur UK, US & Europe Distribution

Host : Llama

Reactivity : N/A

Tested Application : E, WB, IF, Flow

Application: Llama IgG2 FITC conjugated isotype control can be utilized in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

Isotype: IgG2

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Concentration: 1 mg/mL

Storage Condition: Store vial at 4˚C prior to restoration. For extended storage aliquot contents and freeze at -20˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Restore with 100 uL deionized water (or equivalent)

Background: Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.