Description

Copper assay kit (3,5-DiBr-PAESA)

[ Product Description ]

Inside human body, approximately 95% of copper in the plasma is attached to alpha-2- globulin, ceruloplasmin and some enzymes with ferroxidase activity. The main role of protein containing copper is oxidation-reduction function. Copper deficiency is known to be associated with some diseases such as heart disease, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease.

It has been also reported that copper deficiency may cause reduction of anti-oxidative function in vivo. On the other hand, the excess copper is toxic. This kit is a simple colorimetric assay which can detect copper precisely in biological fluids in a short period of time (about 10 minutes).

[ Assay principle ]

In this assay system, copper is dissociated from ceruloplasmin and other copper containing proteins, by denaturing agent in acidic condition.

Free and protein-associated copper will form a copper -3-5 DiBr-PAESA complex, which can be detected by absorbance at 580 nm.

[ Performance ]

1. Sensitivity:
2. Accuracy:
3. Intra assay variation:
4. Assay range:

O.D. at 200 µg/dL standard will be 0.03 - 0.1
±10% of known concentration
C.V. is less than 5%
3 - 400 µg/dL

[ Specifications ]

Assay principle:	DiBr-PAESA method (Colorimetric assay at 580 (570-590) nm)
Specifity:	Specific to Cu²⁺ and Cu⁺
Measuring range:	3 - 400 µg/dL
Time to answer:	10 minutes
Applications:	Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Will be interfered by EDTA.
Storage temperature:	2 - 8°C (don't freeze).
Expiry date:	1 year after the day of manufacturing.
Required but not provided:	Distilled water 96 well microtiter plate Micropipettors and pipette tips Microtiter plate reader (measuring wavelength 580 nm)

[ Contents of this kit ]

R-1 Buffer (ready to use):	24 mL
R-2 Chelate color (ready to use):	0.5 mL
Copper standard 200 µg/dL (ready to use):	1.2 mL

[ References: Assay examples ]

1)	Kazuma Nakanishi, Masahiro Tomita and Katsuya Kato: Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture. J Asian Ceramic Societies 3(1), p70-76(2015) doi:10.1016/j.jascer.2014.10.011
2)	Nobuhiko Asada, Aoi Kedamori, Yumiko Kusano and Tetsuro Takeuchi: Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes. J Life Sci 8(6),p517-521(2014)
3)	Yasuyuki Sakurai, Itsuki Anzai, and Yoshiaki Furukawa: A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase. J Biol Chem 289(29),p20139-20149(2014). doi: 10.1074/jbc.M114.567677
4)	Sakamoto, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K: Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor. Int J Biochem Cell Biol. 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010

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Additional Information

Size:	100 Tests
Type of Marker:	Trace elements assay
Marker:	Zn
Storage:	2 - 8°C (don't freeze).
Colorimetric:	550-580 nm
Assay range:	4-1,000 ug/dL
Usage:	Urine, Serum, Tissue
Remark:	Can detect total (free and protein - bond) zinc. Also applicable to extractes from tissues, plants and hair.