Cortisol ISWE Mini-Kit - ISWE002

The ISWE Cortisol Mini-Kit is specifically designed to measure Cortisol present in a variety of samples and species for wildlife conservation. The end user must provide or coat their own anti-rabbit IgG secondary antibody coated plates, in addition to providing assay buffer, wash buffer, substrate and stop solution. See Related Products for suitable options available.

NOTE: The 50X Concentrate Rabbit Antibody to Cortisol cannot be directly coated onto plates for EIA use. It is an unpurified serum diluted in a buffer containing proteins and detergents. The antibody WILL NOT bind to uncoated microtiter plates effectively. The end user must use plates coated with goat or donkey anti-rabbit IgG.

Kits Include:

ISWE Cortisol Antibody Concentrate

ISWE Cortisol Conjugate Concentrate

Cortisol Standard

Storage Instructions

All components of this kit should be aliquoted into vials upon first thaw. Avoid repeat freeze-thaw cycles.

Store at -20°C until the expiration date of the kit.

Protocols

The rabbit anti-Cortisol is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable assay buffer. If your assay buffer has protein, detergent and preservative in it, the diluted antibody will be stable for greater than 1 month at 4°C. Please see Related Products for a suitable Assay Buffer.

The Cortisol-HRP conjugate is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable diluent or assay buffer. If you wish to store the conjugate diluted for greater than 1 month at 4°C you should dilute it in a suitable conjugate diluent. Please see Related Products for a suitable Conjugate Diluent.

The Cortisol standard stock is provided at 320 ng/mL (320,000 pg/mL) in a special storage solution.  The solution contains an organic solvent. Prerinse the pipet tip several times to ensure accurate delivery.

NOTE: The dilution of the ISWE Mini-kit antibody and conjugate are such that when diluted 1:50 in Assay Buffer or Conjugate Diluent and added to an ’ antibody coated plate, using 100 μL of sample or standards, 50 μL of conjugate and 50 μL of antibody in a 2 hour room temperature with shaking incubation, followed by our TMB as substrate, these reagents will generate approximately 1.0 OD at 450 nm. YOUR RESULTS WILL VARY DEPENDING ON A NUMBER OF VARIABLES. You will have to titer the antibody or conjugate to obtain the optimum conditions for your assay system.

Cortisol ISWE Mini-Kit - ISWE002 - DataSheet